2-[4-(4-Methylphenylsulfonyl)piperazin-1-yl]-1-(4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl)ethanone

In the title thienopyridine derivative, C20H25N3O3S2, the piperazine ring exhibits a chair conformation and the tetra­hydro­pyridine ring exhibits a half-chair conformation. The folded conformation of the mol­ecule is defined by the N—C—C—N torsion angle of −70.20 (2) °. Inter­molecular C—H⋯S and C—H⋯O hydrogen bonds help to establish the packing

Eosinophilia triggers changes in IL-5, eotaxin and IL-17, and acts as a prognostic biomarker for atopic dermatitis

Purpose: To investigate the implication of eosinophilia in atopic dermatitis (AD).Methods: A total of 139 AD patients from The First Affiliated Hospital of Guangxi University of Chinese Medicine between February 2013 and May 2015, were involved in this study. Scoring atopic dermatitis (SCORAD) index was used to evaluate the skin lesions. The levels of IL-4, IL-5, IL-13, IL-17, INFgamma, IP-10, eotaxin and Regulated on Activation, Normal T-cell Expressed and Secreted (RANTES), were determined with commercial enzyme-linked immunosorbent assay (ELISA) kits. Eosinophil counts were carried out by granulocyte count method. Correlation between SCORAD scores and levels of cytokines was analyzed using the Spearman correlation method.Results: SCORAD scores significantly increased in the eosinophil-positive group when compared to eosinophil-negative group (p < 0.05). Eosinophil counts correlated with SCORAD scores in the eosinophil-positive group (p < 0.05). INF-γ, IP-10 and RANTES levels were significantly higher in the eosinophil-positive group than in eosinophil- negative group, while IL-5, eotaxin and IL-17 levels significantly decreased in eosinophil-positive group (p < 0.05). In the eosinophil-positive group, IL-5, eotaxin and IL-17 levels positively correlated with SCORAD scores.Conclusion: Eosinophilia triggers lL-5, eotaxin and IL-17 changes and acts as a prognostic biomarker for atopic dermatitis. These findings may give further insights into the pathogenesis of AD.Keywords: Atopic dermatitis, Eosinophilia, SCORAD score, Biomarker, Cytokine

Fever Screening at Airports and Imported Dengue

Airport fever screening in Taiwan, July 2003–June 2004, identified 40 confirmed dengue cases. Results obtained by capture immunoglobulin (Ig) M and IgG enzyme-linked immunoassay, real time 1-step polymerase chain reaction, and virus isolation showed that 33 (82.5%) of 40 patients were viremic. Airport fever screening can thus quickly identify imported dengue cases

Identify submitochondria and subchloroplast locations with pseudo amino acid composition: Approach from the strategy of discrete wavelet transform feature extraction

AbstractIt is very challenging and complicated to predict protein locations at the sub-subcellular level. The key to enhancing the prediction quality for protein sub-subcellular locations is to grasp the core features of a protein that can discriminate among proteins with different subcompartment locations. In this study, a different formulation of pseudoamino acid composition by the approach of discrete wavelet transform feature extraction was developed to predict submitochondria and subchloroplast locations. As a result of jackknife cross-validation, with our method, it can efficiently distinguish mitochondrial proteins from chloroplast proteins with total accuracy of 98.8% and obtained a promising total accuracy of 93.38% for predicting submitochondria locations. Especially the predictive accuracy for mitochondrial outer membrane and chloroplast thylakoid lumen were 82.93% and 82.22%, respectively, showing an improvement of 4.88% and 27.22% when other existing methods were compared. The results indicated that the proposed method might be employed as a useful assistant technique for identifying sub-subcellular locations. We have implemented our algorithm as an online service called SubIdent (http://bioinfo.ncu.edu.cn/services.aspx)